sirius red fast green collagen staining kit  (Chondrex Inc)

Journal: International Journal of Oncology

Article Title: RNA-binding protein DAZAP1 promotes gastric cancer metastasis by enhancing NOTCH1 and JAG1 mRNA stability

doi: 10.3892/ijo.2026.5863

Figure Lengend Snippet: DAZAP1 binds NOTCH1 or JAG1 mRNA in GC cells. (A) DAZAP1-binding motif was predicted using the RBPmap database ( rbpmap.technion.ac.il/ ). (B) Overlap between DAZAP1-interacting genes identified using RIP-seq and EMT-related genes from the EMT database ( emtome.org ). (C) Quantification of target genes involved in the pathways in the cancer signaling pathway, including NOTCH1 and JAG1, according to KEGG analysis in the WebGestalt database ( webgestalt.org/ ). (D) RT-qPCR analysis of the mRNA levels of NOTCH1 and JAG1 following DAZAP1 knockdown in AGS and MKN-45 cells. RT-qPCR analysis of (E) NOTCH1 and (F) JAG1 with anti-DAZAP1 and IgG control antibodies in AGS cells. (G) NOTCH1 and (H) JAG1 mRNA t 1/2 in DAZAP1 knockdown AGS cells. n=3. (I) Verification of full-length or truncated DAZAP1 plasmids using western blotting and anti-FLAG antibody. RIP assay analysis of (J) NOTCH1 and (K) JAG1 mRNA binding to the DAZAP1 structure domains. *** P<0.01 and **** P<0.001 vs. scr-siRNA. RRM, RNA recognition motif; CTD, C-terminal domain; DAZAP1, deleted in azoospermia-associated protein 1; RIP-seq, RNA Immunoprecipitation-sequencing; EMT, Epithelial-Mesenchymal Transition; KEGG, Kyoto Encyclopedia of Genes and Genomes; RT-q, Reverse Transcription quantitative real-time; t 1/2 , half-life; scr, scramble; si, small interfering; F, Fragment; FL, Full-Length; aa, amino acid.

Article Snippet: RT was performed in strict accordance with the manufacturer's instructions for the was conducted via Hieff ® Fast Cell SYBR Green RT-qPCR Kit (cat. no. 11172ES40, Shanghai Yeasen Biotechnology Co., Ltd.).

Techniques: Binding Assay, Quantitative RT-PCR, Knockdown, Control, Western Blot, RNA Immunoprecipitation, Sequencing, Reverse Transcription

Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

doi: 10.1093/gerona/glag085

Figure Lengend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

Article Snippet: The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups.

Techniques: Staining